![]() Was this page helpful? Please let us know. Tri-color Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western. Although Image Studio Software is no longer in development, we continue to sell and support it. Image Studio™ Softwareįor instructions on entering marker molecular weights and sizing bands in Image Studio Software, visit /bio/support. For more information, see the instructions for the Experiment workflow you are using at /WesternWorkflowOverview. ![]() ![]() Molecular weights for WesternSure Chemiluminescent Pre-Stained Protein ladders can be sized in Empiria Studio Software. The WesternSure Pre-stained Chemiluminescent Protein Ladder is not a standard and molecular weights may vary, depending on the gel type. Stripping buffer alters the chemiluminescent functionality of the ladder, resulting in weak or no signal. The ladder is not recommended for use when stripping and reprobing Western blots. Mix the product thoroughly before use to dissolve any solids that may have precipitated during storage. Bring the product to room temperature while protecting from light. WesternSure Pre-stained Chemiluminescent Protein Ladders are provided ready to use in loading buffer with no dilution, heating, or reconstitution required. To see a complete portfolio of LI-COR molecular weight markers, visit /proteinmarkers. The ladder is optimized for use with Bis-Tris and Tris-Glycine systems. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest. In gels, the ladder can be used to visualize progress of the protein separation during electrophoresis and to estimate the molecular weight of unknown proteins based on their relative mobility. The ladder offers both pre-stained and chemiluminescent functionalities and is suitable for use with film and a variety of chemiluminescent substrates. It is concluded that the estimated molecular weight of SMOBIO’s pre-stained marker shows a curve matching well with that of unstained native proteins (MARK12), representing a good estimation of the MW of each pre-stained protein in the SDS-PAGE analysis.The WesternSure Pre-stained Chemiluminescent Protein Ladder provides a ladder of convenient and consistent protein sizes (8 - 260 kDa) for use with polyacrylamide gels and on Western membranes where chemiluminescent detection is used. Although it is impossible to define "precision" for molecular weight of proteins in SDS-PAGE, we did compare the migration pattern of pre-stained markers with unstained protein marker (Invitrogen MARK12) for calibration. Therefore, in the product description, we suggest our users to calibrate the MW against their interested proteins. We did compare the migration patterns of SMOBIO’s Protein Markers with other brands, and we concluded that it was difficult to define “precision” due to the reasons mentioned above. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer. For example, a protein which is highly hydrophilic might show a particular higher position in the SDS-PAGE analysis when compared to a hydrophobic one. It is known that the analysis of protein size by an SDS-PAGE is only for “estimation” because of the intrinsic variation of amino acid composition in all proteins including stained and non-stained ones. Usually, pre-stained marker is written on “estimated molecular weight” for caution. MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. ![]() Under suggested conditions, PM5200 ExcelBand™ 3-color Pre-Stained Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE ( Tris-Glycine, Three reference bands - 75 kDa (red), 40 kDa (green), and 20 kDa (blue)Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25☌), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Ready-to-use - Premixed with a loading buffer for direct loading, no need to boil. The ladder also works for Fluorescent western blot developing. The PM5200 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Its very useful that the pattern is assymetric to allow for easy reading. Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5200 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). ![]()
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